Acetylcholine in the Electric Organ of Torpedo

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Identification of the acetylcholine receptor subunit in the lipid bilayer of Torpedo electric organ excitable membranes.

The polypeptides of acetylcholine receptor-rich membrane fragments (from the electric organ of Torpedo californica), which are embedded or span the lipid bilayer, have been identified by their photolabeling with the lipid-soluble reagent, 5-[’251]iodonaphthyl-l-azide. This reagent has been shown previously (Bercovici, T., and Gitler, C. (1978) Biochemistry 17, 1484-1489) to label only those pro...

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Rapid acetylcholine and adenosine triphosphate oscillations triggered by stimulation of the Torpedo electric organ.

1. When the electric organ of Torpedo is stimulated a large number of synchronized cholinergic synapses are activated. This permits the measurement of changes in the tissue level of ACh associated with the release process, usually recorded as an electric discharge. 2. At 5 Hz stimulation the output per impulse and the amount of cytoplasmic (free ACh) declines for about 30 s. The output then rem...

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The Fine Structure of the Electric Organ of Torpedo Marmorata

The fine structure of the electric organ of the fish Torpedo marmorata has been examined after osmium tetroxide or potassium permanganate fixation, acetone dehydration, and Araldite embedment. This organ consists of stacks of electroplaques which possess a dorsal noninnervated and a ventral richly innervated surface. Both surfaces are covered with a thin basement membrane. A tubular membranous ...

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The Release of Phosphate from the Electric Organ of Torpedo

The electroplates of the electric organ of Torpedo are embryonically derived from striated muscle fibres which lose their ability to contract and develop greatly expanded end-plate surfaces (cf. Rosenberg, 1928). From consideration of the thermodynamics of the Torpedo organ discharge, Bernstein (1912) concluded that the electric energy of the discharge was derived from a difference between the ...

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Consequences of alkaline treatment for the ultrastructure of the acetylcholine-receptor-rich membranes from Torpedo marmorata electric organ

After fixation with glutaraldehyde and impregnation with tannic acid, the membrane that underlies the nerve terminals in Torpedo marmorata electroplaque presents a typical asymmetric triple-layered structure with an unusual thickness; in addition, it is coated with electron-dense material on its inner, cytoplasmic face. Filamentous structures are frequently found attached to these "subsynaptic ...

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ژورنال

عنوان ژورنال: Nature

سال: 1965

ISSN: 0028-0836,1476-4687

DOI: 10.1038/2071295a0